Coding

Part:BBa_K1396001:Design

Designed by: Ariel Hernandez-Leyva   Group: iGEM14_Yale   (2014-10-10)


pSB1C3: LL-37_MFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We capped the LL-37 Domain which is otherwise toxic to cells with a removable strep tag that can be used for column chromatography. Additionally there are linker units between individual components


Source

Synthesized in lab.

References